High density cell culture of Panax notoginseng for production of ginseng saponin and polysaccharide in an airlift bioreactor

High cell density of Panax notoginseng in a 17 l airlift bioreactor was achieved in batch cultivation using a modified MS medium. The dry cell weight, ginseng saponin and polysaccharide reached 24, 1.7 and 2.8 g l-1, respectively, after 15 d. A strategy of sucrose feeding based on changes in the specific O2 uptake rate was applied to the cell cultures, which increased these respective yields to 30, 2.3 and 3.2 g l-1.
Keywords: airlift bioreactor; ginseng saponin and polysaccharide; high density cell culture; Panax notoginseng; plant cell culture
Language: English
Document Type: Research article
Affiliations: 1: State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China 2: (Fax: +86-21-64253904 3: E-mail: jjzhong@ecust.edu.cn)
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